New assay for the O-alkyl cleavage enzyme with alkyl-lysophospholipids as substrates.
نویسندگان
چکیده
The O-alkyl cleavage enzyme is important for the metabolism of cytotoxic alkyl-lysophospholipids. We have developed a simple new method for the determination of the enzyme activity which is based on the formation of water-soluble phosphate during the enzyme reaction. This is the first assay which avoids the use of radiolabeled substrates.
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ورودعنوان ژورنال:
- Cancer research
دوره 45 2 شماره
صفحات -
تاریخ انتشار 1985